Histone H3K23me1 antibody (pAb)

RRID: AB_2793238
商品コード: 39387 Format: 200 µl ¥66,800 Buy
商品コード: 39388 Format: 10 µl ¥18,800 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ICC/IF
ChIP-Seq
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H3 monomethyl Lys23 antibody was raised against a peptide containing monomethyl Lys23 of human histone H3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 
Histone H3K23me1 antibody (pAb) tested by Western blot.

Histone H3 monomethyl Lys23 pAb tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with Histone H3 monomethyl Lys23 pAb at a dilution of 1:1,000.

Histone H3K23me1 antibody (pAb) tested by dot blot analysis.

Histone H3 monomethyl Lys23 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys23 pAb for monomethyl Lys23 histone H3. Methylated peptides corresponding to the immunogen and related sequences derived from histone H3 were spotted onto PVDF and probed with the antibody at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Top row: Lane 1: Unmod H3 aa 1-10 peptide. Lane 2: Monomethyl lysine 4. Lane 3: Dimethyl lysine 4. Lane 4: Trimethyl lysine 4. Lane 5: Unmodified H3 aa 6-22 peptide. Lane 6: Monomethyl lysine 9. Lane 7: Dimethyl lysine 9. Lane 8: Trimethyl lysine 9.
Bottom row: Lane 1: Dimethyl lysine 14. Lane 2: Monomethyl lysine 18. Lane 3: Dimethyl lysine 18. Lane 4: Trimethyl lysine 18. Lane 5: Unmod H3 aa 18-27 peptide. Lane 6: Monomethyl lysine 23. Lane 7: Dimethyl lysine 23. Lane 8: Trimethyl lysine 23. Lane 9: Unmod H3 aa 22-32 peptide. Lane 10: Monomethyl lysine 27. Lane 11: Dimethyl lysine 27. Lane 12: Trimethyl lysine 27.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting