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Histone H2AT120ph antibody (pAb)

商品コード: 39391 Format: 200 µl ¥67,000 Buy Now
商品コード: 39392 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:16 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Dot Blot Validated

for Histone H2A phospho Thr120 antibody (pAb)Application Notes


Validated Applications:
WB: 1:2,500 - 1:10,000 dilution

for Histone H2A phospho Thr120 antibody (pAb)Immunogen

This Histone H2A phospho Thr120 antibody was raised against a peptide containing phospho-threonine 120 of histone H2A.

for Histone H2A phospho Thr120 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 61195) of this antibody that was purified by Protein A Chromatography is also available.

for Histone H2A phospho Thr120 antibody (pAb)References

 
Histone H2AT120ph antibody (pAb) tested by Western blot.

Histone H2A phospho Thr120 pAb tested by Western blot.
HeLa acid extract (20 µg per lane) was probed with Histone H2A phospho Thr120 pAb at a dilution of 1:2,500.
    Lane 1: No treatment.
    Lane 2: Cells treated with colcemid to arrest cells at mitosis.

Histone H2AT120ph antibody (pAb) tested by dot blot analysis.

Histone H2A phospho Thr120 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2A phospho Thr120 pAb for phospho Thr120 histone H2A. The modified and unmodified peptides to the immunogen were spotted onto PVDF and probed with the antibody at a dilution of 1:2,500. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Phospho Thr120 peptide.
    Lane 2: Unmodified Thr120 peptide.

for Histone H2A phospho Thr120 antibody (pAb)Background

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Phosphorylation of histones occurs at multiple sites during mitosis. H2A Thr120 phosphorylation is observed on chromatin during both mitosis and meiosis. Thr120 phosphorylation is inversely correlated with ubiquitylation of H2A Lys119 in meiotic mouse spermatocytes. In Drosophila, loss of H2A Thr120 phosphorylation is associated with a failure to disassemble the synaptonemal complex, impaired loading of condensin and female infertility. It is possible that H2A Thr120 phosphorylation is involved in the regulation of chromatin structure.

for Histone H2A phospho Thr120 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H2A phospho Thr120 antibody.

for Histone H2A phospho Thr120 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2A phospho Thr120 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H2AT120ph antibody (pAb)
 

Data Thumbnails

Histone H2AT120ph antibody (pAb) tested by Western blot.

Western blot of Histone H2AT120ph pAb.
(Click image to enlarge and see details.)

Histone H2AT120ph antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H2AT120ph pAb.
(Click image to enlarge and see details.)