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alpha Tubulin antibody (mAb)
| Clone: 5-B-1-2 |
| Catalog No: 39527 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39528 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:1,000 - 1:10,000 | — | 1:500 - 1:2,500 |
for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Background
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of α-tubulin and β-tubulin. Alpha Tubulin expression is reduced in cells deficient in MeCP2, leading to conditions such as Rett syndrome and autistic-spectrum disorders.
for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Immunogen
This antibody was raised against total protein purified from sea urchin sperm axonemes.
for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Application Notes
This alpha Tubulin antibody (Clone 5-B-1-2) has been validated for use in immunofluorescence (1:500 - 1:2,500 dilution) and Western blot (1:1,000 - 1:10,000 dilution). Though raised against sea urchin alpha-tubulin, this antibody recognizes alpha-tubulin in all vertebrate species tested.
for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Buffer
Ascites containing 30% glycerol and 0.035% sodium azide.
for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for alpha Tubulin antibody (Clone 5-B-1-2).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with alpha Tubulin antibody (mAb).
for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)References
The development and use of this antibody is described in Piperno and Fuller (1985) J Cell Biol 101:2085 and Piperno et al (1987) J Cell Biol (1987) 104:289.
alpha Tubulin mAb (Clone 5-B-1-2) tested by Western blot.
HeLa whole-cell extract (15 µg/lane) probed with alpha Tubulin mAb (1:5,000 dilution).
alpha Tubulin mAb (Clone 5-B-1-2) tested by immunofluorescence.
Top: HeLa cells stained with alpha Tubulin mAb at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain™ Immunofluorescence Tools.
for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for alpha Tubulin antibody (mAb) (Clone 5-B-1-2)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
