HDAC1 antibody (mAb)

for HDAC1 antibody (mAb) (Clone 10E2)Background

HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

for HDAC1 antibody (mAb) (Clone 10E2)Immunogen

This HDAC1 antibody was raised against a KLH-conjugated peptide corresponding to amino acids 467-482 of human and murine HDAC1.

for HDAC1 antibody (mAb) (Clone 10E2)Application Notes

This HDAC1 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and chromatin IP (20 µl per ChIP), and it has been reported to work in immunofluorescence, immunohistochemistry and immunoprecipitation.

for HDAC1 antibody (mAb) (Clone 10E2)Buffer

Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide.

for HDAC1 antibody (mAb) (Clone 10E2)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HDAC1 antibody (Clone 10E2).

for HDAC1 antibody (mAb) (Clone 10E2)References

This antibody was used in Lagger et al (2002) EMBO J 21:2672 and in Lagger et al (2003) Mol Cell Biol 23:2669.

for HDAC1 antibody (mAb) (Clone 10E2)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HDAC1 antibody (mAb) (Clone 10E2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.