Nucleolin antibody (mAb)

for Nucleolin antibody (mAb) (Clone 3G4B20)Background

Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.

for Nucleolin antibody (mAb) (Clone 3G4B20)Immunogen

This Nucleolin antibody was raised against a salt-extracted nuclear matrix preparation from MDCK cells.

for Nucleolin antibody (mAb) (Clone 3G4B20)Application Notes

This Nucleolin antibody has been validated for use in immunofluorescence (1:500 dilution) and Western blotting (1:500 - 1:2,000 dilution).

for Nucleolin antibody (mAb) (Clone 3G4B20)Buffer

Concentrated culture supernatant.

for Nucleolin antibody (mAb) (Clone 3G4B20)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Nucleolin antibody (Clone 3G4B20).

for Nucleolin antibody (mAb) (Clone 3G4B20)References

This antibody was used in Gotzmann et al (1997) Electrophoresis 18:2645.

for Nucleolin antibody (mAb) (Clone 3G4B20)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Nucleolin antibody (mAb) (Clone 3G4B20)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.