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SNF2h antibody (mAb)
| Catalog No: 39543 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39544 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 1:500 - 1:2,000 | Yes | Yes | Yes |
for SNF2h antibody (mAb)Background
SNF2h is a human member of the SWI/SNF superfamily. SNF2h is the catalytic subunit of several chromatin remodeling complexes, such as CHRAC, RSF, ACF, NuRD and NoRC. SNF2H is a member of the SWI/SNF superfamily of proteins and members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SNF2h is a component of the chromatin remodeling and spacing factor RSF (Rsf1), a facilitator of the transcription of class II genes by RNA pol II. It is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
for SNF2h antibody (mAb)Immunogen
This SNF2h antibody was raised against purified human RSF complex which was used to immunize mice.
for SNF2h antibody (mAb)Application Notes
This SNF2h antibody has been validated for use in Western blotting (1:500 - 1:2,000), and it has been reported to work in chromatin IP, immunofluorescence and immunoprecipitation.
for SNF2h antibody (mAb)Buffer
Ascites containing 30% glycerol and 0.035% sodium azide.
for SNF2h antibody (mAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for SNF2h antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with SNF2h antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use SNF2h antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SNF2h antibody (mAb).
SNF2h mAb tested by Western blot.
HeLa acid extract (10 µg) was probed with SNF2h mAb (1:2,000 dilution).
for SNF2h antibody (mAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for SNF2h antibody (mAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
