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Tools to analyze nuclear function,
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Antibodies

JunB antibody (pAb)

Catalog No: 39549 Format: 200 µl $340 Buy Now
Catalog No: 39550 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:Serum
Purification:None
Applications:WB
Reactivity:Human
Molecular Weight:42 kDa

Applications Table

Western Blot Chromatin IP
1:500 - 1:2,500

for JunB antibody (pAb)Background

JunB (Jun B proto-oncogene) is a member of the Jun family and forms a component of the AP-1 transcription factor complex. JunB forms heterodimers and homodimers with other members of the Jun/Fos family. It is a hallmark of many lymphomas and stimulates proliferation. Other AP-1 family members consist of c-Fos, Fra-1, Fra-2, FosB, c-Jun, and JunD.

for JunB antibody (pAb)Immunogen

This JunB antibody was raised against a synthetic peptide corresponding to amino acids 28-42 of human JunB.

for JunB antibody (pAb)Application Notes

This JunB antibody has been validated for use in Western blotting (1:500 - 1:2,500 dilution). Individual optimization may be required.

for JunB antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for JunB antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JunB antibody.

 
JunB pAb tested by Western blot.

JunB pAb tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with JunB pAb at a dilution of 1:1,000.

for JunB antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for JunB antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

JunB antibody (pAb)
 

Data Thumbnails

JunB pAb tested by Western blot.

Western blot of JunB pAb.
(Click image to enlarge and see details.)