Your Cart
Browse
Filter
p53 antibody (mAb)
| Clone: DO1 |
| Catalog No: 39553 | Format: 200 µg | $340 | Buy Now |
| Catalog No: 39554 | Format: 10 µg | $70 | Buy Now |
|
|
Applications Table
| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 0.5 - 2 µg/ml | — | 5 µg per IP |
for p53 antibody (mAb) (Clone DO1)Background
p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, a ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.
for p53 antibody (mAb) (Clone DO1)Immunogen
This p53 antibody was raised against full-length recombinant human p53 protein.
for p53 antibody (mAb) (Clone DO1)Application Notes
This p53 antibody has been validated for use in Western blotting (0.5 - 2.0 µg/ml dilution) and immunoprecipitation (5 µg per IP).
for p53 antibody (mAb) (Clone DO1)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for p53 antibody (mAb) (Clone DO1)Positive Control
Nuclear extracts from U2OS cells treated with Camptothecin can be used as a positive control for p53 antibody (Clone DO1).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use p53 antibody (mAb).
p53 mAb (Clone DO1) tested by Western blot.
5 µg of p53 mAb (Clone DO1) was used to immunoprecipitate p53 from 500 µg of camptothecin-treated U2OS nuclear extract (lane 2). 5 µg of mouse IgG was also used as a control (lane 1). The immunoprecipitated protein was detected by western blotting using the same p53 antibody.
p53 mAb (Clone DO1) tested by Western blot.
Western Blot: Nuclear extract of U2OS cells (20 μg) probed with p53 mAb (Clone DO1) (1 µg/ml).
Lane 1: No treatment.
Lane 2: Cells treated with camptothecin.
for p53 antibody (mAb) (Clone DO1)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for p53 antibody (mAb) (Clone DO1)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
