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Histone H3 acetyl Lys79 antibody (pAb)
| Catalog No: 39565 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39566 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | 5 - 10 µl per ChIP |
for Histone H3 acetyl Lys79 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation.
for Histone H3 acetyl Lys79 antibody (pAb)Immunogen
Peptide containing acetyl-lysine 79 of human histone H3.
for Histone H3 acetyl Lys79 antibody (pAb)Application Notes
This Histone H3 acetyl Lys79 antibody (H3K79Ac) has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and chromatin IP (5 - 10 µl per ChIP).
for Histone H3 acetyl Lys79 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for Histone H3 acetyl Lys79 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys79 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 acetyl Lys79 antibody (pAb).
Histone H3 acetyl Lys79 pAb tested by Western blot.
HeLa acid extract (40 µg per lane) was probed with Histone H2B acetyl Lys46 polyclonal antibody (1:1,000).
Lane 1: No treatment.
Lane 2: Cells treated with sodium butyrate. An unidentified band is also observed at 80 kDa.
Histone H3 acetyl Lys79 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys79 pAb for acetyl Lys79 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: Unmodified amino acids 75-83 of histone H3.
Lane 2: Acetyl-Lys79 peptide.
Lane 3: Acetyl-Lys14 peptide.
Lane 4: Acetyl-Lys9 peptide.
Lane 5: Acetyl-Lys18 peptide.
Lane 6: Acetyl-Lys23 peptide.
Lane 7: Acetyl-Lys27 peptide.
Lane 8: Acetyl-Lys37 peptide.
Lane 9: Acetyl-Lys36 peptide.
Lane 10: Acetyl-Lys5 H2A peptide.
Lane 11: Acetyl-Lys9 H2A peptide.
Histone H3 acetyl Lys79 pAb tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and 50 µl of Ready-to-ChIP HeLa Chromatin (Catalog No. 53015) per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR.
Lane 1: ChIP using negative control rabbit IgG.
Lane 2: ChIP using 10 µl of Histone H3 acetyl Lys79 pAb.
Lane 3: PCR input control.
Histone H3 acetyl Lys79 pAb tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of Histone H3 acetyl Lys79 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for Histone H3 acetyl Lys79 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 acetyl Lys79 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
