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Histone H2BK46me2 antibody (pAb)

商品コード: 39567 Format: 200 µl ¥67,000 Buy Now
商品コード: 39568 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:15 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H2B dimethyl Lys46 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution
ICC/IF: 1:250 - 1:1,000 dilution

Published Applications:
WB

for Histone H2B dimethyl Lys46 antibody (pAb)Immunogen

This Histone H2B dimethyl Lys46 antibody was raised against a peptide containing dimethyl-lysine 46 of human histone H2B.

for Histone H2B dimethyl Lys46 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H2B dimethyl Lys46 antibody (pAb)References

 
Histone H2BK46me2 antibody (pAb) tested by immunofluorescence.

Histone H2B dimethyl Lys46 pAb tested by immunofluorescence.
Top left: HeLa cells stained with Histone H2B dimethyl Lys46 pAb (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.

Histone H2BK46me2 antibody (pAb) tested by Western blot.

Histone H2B dimethyl Lys46 pAb tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H2B dimethyl Lys46 pAb (1:2,000 dilution).

Histone H2BK46me2 antibody (pAb) tested by dot blot analysis.

Histone H2B dimethyl Lys46 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2B dimethyl Lys46 pAb for dimethyl Lys46 histone H2B. Methylated peptides corresponding to the immunogen and related sequences derived were spotted onto PVDF and probed with the antibody at 1:20,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: unmodified histone H2B peptide.
    Lane 2: monomethyl Lys46 H2B peptide.
    Lane 3: dimethyl Lys46 H2B peptide.
    Lane 4: trimethyl Lys46 H2B peptide.
    Lane 5: dimethyl Lys4 H3 peptide.

for Histone H2B dimethyl Lys46 antibody (pAb)Background

Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Changes in methylation of histone H2B seem to be involved in heat-shock mechanisms.

for Histone H2B dimethyl Lys46 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H2B dimethyl Lys46 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2BK46me2 antibody (pAb).

for Histone H2B dimethyl Lys46 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2B dimethyl Lys46 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H2BK46me2 antibody (pAb)
 

Data Thumbnails

Histone H2BK46me2 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H2BK46me2 pAb.
(Click image to enlarge and see details.)

Histone H2BK46me2 antibody (pAb) tested by Western blot.

Western blot of Histone H2BK46me2 pAb.
(Click image to enlarge and see details.)

Histone H2BK46me2 antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H2BK46me2 pAb.
(Click image to enlarge and see details.)