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Histone H2B dimethyl Lys46 antibody (pAb)
| Catalog No: 39567 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39568 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:500 - 1:2,000 | — | 1:250 - 1:1,000 |
for Histone H2B dimethyl Lys46 antibody (pAb)Background
Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Changes in methylation of histone H2B seem to be involved in heat-shock mechanisms.
for Histone H2B dimethyl Lys46 antibody (pAb)Immunogen
Peptide containing dimethyl-lysine 46 of human histone H2B.
for Histone H2B dimethyl Lys46 antibody (pAb)Application Notes
This Histone H2B dimethyl Lys46 antibody (H2BK46me2) has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and immunofluorescence (1:250 - 1:1,000 dilution).
for Histone H2B dimethyl Lys46 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for Histone H2B dimethyl Lys46 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H2B dimethyl Lys46 antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2B dimethyl Lys46 antibody (pAb).
Histone H2B dimethyl Lys46 pAb tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H2B dimethyl Lys46 pAb (1:2,000 dilution).
Histone H2B dimethyl Lys46 pAb tested by immunofluorescence.
Top left: HeLa cells stained with Histone H2B dimethyl Lys46 pAb (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.
Histone H2B dimethyl Lys46 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2B dimethyl Lys46 pAb for dimethyl Lys46 histone H2B. Methylated peptides corresponding to the immunogen and related sequences derived were spotted onto PVDF and probed with the antibody at 1:20,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified histone H2B peptide.
Lane 2: monomethyl Lys46 H2B peptide.
Lane 3: dimethyl Lys46 H2B peptide.
Lane 4: trimethyl Lys46 H2B peptide.
Lane 5: dimethyl Lys4 H3 peptide.
for Histone H2B dimethyl Lys46 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H2B dimethyl Lys46 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
