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Histone H2B acetyl Lys46 antibody (pAb)
| Catalog No: 39571 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39572 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:4,000 - 1:10,000 | 5 - 10 µl per ChIP |
for Histone H2B acetyl Lys46 antibody (pAb)Background
Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.
for Histone H2B acetyl Lys46 antibody (pAb)Immunogen
Peptide including acetyl-lysine 46 of human histone H2B.
for Histone H2B acetyl Lys46 antibody (pAb)Application Notes
This Histone H2B acetyl Lys46 antibody (H2BK46Ac) has been validated for use in Western blotting (1:4,000 - 1:10,000 dilution) and chromatin IP (5 - 10 µl per ChIP).
for Histone H2B acetyl Lys46 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for Histone H2B acetyl Lys46 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H2B acetyl Lys46 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2B acetyl Lys46 antibody (pAb).
Histone H2B acetyl Lys46 pAb tested by Western blot.
Acid extract of HeLa cells (20 µg per lane) was probed with Histone H2B acetyl Lys46 polyclonal antibody (1:4,000 dilution).
Lane 1: Untreated cells.
Lane 2: Cells treated with sodium butyrate.
Lane 3: Recombinant Histone H2B (200 ng).
Histone H2B acetyl Lys46 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys46 pAb for acetyl Lys46 histone H2B. Decreasing amounts of acetylated peptides corresponding to the immunogen and related sequences were spotted onto PVDF and probed with the antibody at 1:4,000.
Lane 1: acetyl Lys46 histone H2B peptide.
Lane 2: unmodified Lys46 histone H2B peptide.
No detection of peptides (acetylated) corresponding to lysine 9, 14, 18, 23, 27, and 56 of Histone H3 was observed with Histone H2B acetyl Lys46 pAb.
In addition, no detection of peptides (acetylated) corresponding to lysine 5, 15, 16, and 120 of Histone H2B was observed with Histone H2B acetyl Lys46 pAb.
Histone H2B acetyl Lys46 pAb tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and 50 µl of Ready-to-ChIP HeLa Chromatin (Catalog No. 53015) per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR.
Lane 1: ChIP using negative control rabbit IgG.
Lane 2: ChIP using 10 µl of Histone H2B acetyl Lys46 pAb.
Lane 3: PCR input control.
for Histone H2B acetyl Lys46 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H2B acetyl Lys46 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
