Rsf1 antibody (pAb)

for Rsf1 antibody (pAb)Background

Rsf-1 (Remodeling and spacing factor 1) is a member of RSF complex. This complex consists of two subunits: Rsf-1 and SNF2H. RSF possesses a chromatin remodeling activity and a chromatin assembly activity. Rsf-1 assembles the nucleosomes randomly with its histone chaperone activity. The resulting nucleosomes are then redistributed by the ATP-utilizing nucleosome mobilization factor SNF2H into a periodic array. Rsf-1 can be involved in transcriptional repression when it is recruited to DNA via the DNA binding of Gal4 or in transcriptional coactivation when associated with hepatitis B virus pX protein (HBX). In response to hepatitis B infection, Rsf-1 can bind to the viral transcription activator pX, which then coactivates the transcription factor NFκB, thus supporting the life cycle of the viral genome. Additionally, Rsf-1 is thought to be a potent oncogene, with elevated expression observed in ovarian carcinomas.

for Rsf1 antibody (pAb)Immunogen

This Rsf1 antibody was raised against a His-tagged fusion protein corresponding to amino acids 165-350 of Drosophila Rsf1.

for Rsf1 antibody (pAb)Application Notes

This Rsf1 antibody has been validated for use in Western blotting (1:1,000 - 1:5,000 dilution), immunofluorescence (1:1,000 dilution) and immunoprecipitation (10 µl per IP). Addition of 0.05% Tween 20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.

for Rsf1 antibody (pAb)Buffer

PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol.

for Rsf1 antibody (pAb)Positive Control

Active Motif's Schneider's Drosophila L2 nuclear extract (Catalog No. 36087) can be used as a positive control for Rsf1 antibody.

for Rsf1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Rsf1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.