Histone H3 acetyl Lys18 antibody (pAb)

Applications Table

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 acetyl Lys18 antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 acetyl Lys18 antibody (pAb).

Histone H3 acetyl Lys18 antibody tested by Western blot.
A549 whole-cell extract (20 µg per lane) probed with Histone H3 acetyl Lys18 antibody (1:5,000 dilution).
    Lane 1: Untreated cells.
    Lane 2: Cells treated with Trichostatin A.

Histone H3 acetyl Lys18 antibody tested by immunofluorescence.
Top left: HeLa cells stained with Histone H3 acetyl Lys18 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.

Histone H3 acetyl Lys18 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 antibody for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: acetyl-Lys4 peptide.
    Lane 2: unmodified Lys4 peptide.
    Lane 3: acetyl-Lys18 peptide.
    Lane 4: unmodified Lys18 peptide.
    Lane 5: acetyl-Lys9 peptide.
    Lane 6: acetyl-Lys14 peptide.
    Lane 7: acetyl-Lys18 peptide (this is a different peptide sequence as the one in lane 3 which also contains acetyl-Lys18).
    Lane 8: acetyl-Lys23 peptide.
    Lane 9: acetyl-Lys27 peptide.

Histone H3 acetyl Lys18 antibody tested by ChIP.

Top Panel:
Chromatin IP performed using the ChIP-IT^® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of Histone H3 acetyl Lys18 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Bottom Panel:
Chromatin IP performed using the ChIP-IT^® Express Kit (Catalog No. 53008) and 50 µl of Ready-to-ChIP HeLa Chromatin (Catalog No. 53015) per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR.
Lane 1: ChIP using negative control rabbit IgG.
Lane 2: ChIP using 10 µl of Histone H3 acetyl Lys18 antibody.
Lane 3: PCR input control.

Application Key

• ChIP = Chromatin Immunoprecipitation;
• DB = Dot Blot;
• ELISA = Enzyme-linked Immunosorbent Assay;
• EMSA = Electrophoretic Mobility Shift Assay
• FACS = Flow Cytometry;
• ICC = Immunocytochemistry;
• IF = Immunofluorescence;
• IHC = Immunohistochemistry;
• IP = Immunoprecipitation;
• MeDIP = Methyl-DNA Immunoprecipitation;
• WB = Western Blotting