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STAT5A/B phospho Ser726/Ser731 antibody (pAb)

Aliases: MGF, STAT5
商品コード: 39597 Format: 200 µl ¥67,000 Buy Now
商品コード: 39598 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:92 kDa
Reactivity:Human

Applications

Western Blot Validated Dot Blot Validated

for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Immunogen

This STAT5A/B phospho Ser726/Ser731 antibody was raised against a peptide containing phospho Ser726 of human STAT5A. Human STAT5B shares the identical sequence surrounding Ser731.

for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for STAT5A/B phospho Ser726/Ser731 antibody (pAb)References

 
STAT5A/B phospho Ser726/Ser731 antibody (pAb) tested by Western blot.

STAT5A/B phospho Ser726/Ser731 pAb tested by Western blot.
Nb2 nuclear extract (20 µg per lane) was probed with STAT5A/B phospho Ser726/Ser731 pAb (1:2,000).
    Lane 1: No treatment.
    Lane 2: Cells treated with prolactin (25 ng/ml for 20 minutes at 37°C).

STAT5A/B phospho Ser726/Ser731 antibody (pAb) tested by dot blot analysis.

STAT5A/B phospho Ser726/Ser731 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of STAT5A/B phospho Ser726/Ser731 pAb for STAT5A/B phospho Ser726/Ser731. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.

for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Background

STAT5A/B (signal transducer and activator of transcription 5) is a member of the STAT family of transcription factors. STATs undergo serine and tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. The STAT5 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNα, IL-10, IL-6, IL-11, IL-12, IL-2, EGF, among others.

for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Positive Control

Active Motif's Nb2 nuclear extract (prolactin stimulated) (Catalog No. 36106) can be used as a positive control for STAT5A/B phospho Ser726/Ser731 antibody.

for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for STAT5A/B phospho Ser726/Ser731 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

STAT5A/B phospho Ser726/Ser731 antibody (pAb)
 

Data Thumbnails

STAT5A/B phospho Ser726/Ser731 antibody (pAb) tested by Western blot.

Western blot of STAT5A/B phospho Ser726/Ser731 pAb.
(Click image to enlarge and see details.)

STAT5A/B phospho Ser726/Ser731 antibody (pAb) tested by dot blot analysis.

Dot blot of STAT5A/B phospho Ser726/Ser731 pAb.
(Click image to enlarge and see details.)