CENP-E antibody (mAb)

RRID: AB_2793278
Clone: 1H12
Aliases: KIF10, CENPE, PPP1R61
Catalog No: 39619 Format: 200 µg ¥60,000 Buy
Catalog No: 39620 Format: 10 µg ¥21,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
320 kDa
Reactivity:
Human, Mouse

Applications

Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ICC/IF: 1 µg/ml dilution

This CENP-E antibody can be used in Western blot to detect CENP-E if the protein has been first enriched by immunoprecipitation.

Immunogen

This CENP-E antibody was raised against full-length human CENP-E protein.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

CENP-E mouse mAb (Clone 1H12) tested by immunofluorescence.
Staining of HeLa cells with CENP-E mouse mAb (Clone 1H12) at a 1 µg/ml dilution (red) and DAPI (blue).

Detection of CENP-E by immunofluorescence.
U2OS cells were stained with CENP-E antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: CENP-E antibody staining. Right panel: merge.

Background

Centrosome-associated protein E (CENP-E) is a kinesin-like protein and an integral component of kinetochore fibers that link centromeres to spindle microtubules. CENP-E localizes to kinetochores throughout all phases of mitotic chromosome movement. After the onset of anaphase it is released, and by telophase becomes bound to interzonal microtubules of the mitotic spindle. It is likely that CENP-E serves as a chromosome motor during anaphase.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CENP-E antibody (Clone 1H12).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting