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Histone H2B ubiquityl Lys120 antibody (mAb)
| Clone: 56 |
| Catalog No: 39623 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39624 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 0.5 - 2 µg/ml | Published. See Application Notes below. |
for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Background
Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Histone H2B is ubiquitylated at lysine 120 in vertebrates. H2B ubiquityl Lys120 is found preferentially associated with the transcribed regions of highly expressed genes. It is possible that H2B ubiquitylation is associated with transcriptional elongation.
for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Immunogen
This Histone H2B ubiquityl Lys120 antibody was raised against a branched peptide corresponding the the ubiquitin conjugation site at Lys120 of human Histone H2B.
for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Application Notes
This Histone H2B ubiquityl Lys120 antibody was validated for use in Western blotting (0.5 - 2 µg per ml) and chromatin immunoprecipitation.
This antibody was described and used in chromatin immunoprecipitation in reference Minsky et al (2008).
for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H2B ubiquityl Lys120 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2B ubiquityl Lys120 antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H2B ubiquityl Lys120 antibody (mAb).
for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)References
This antibody was used as described in Minsky, N. et al (2008) nat Cell Biol 10: 483–488.
Histone H2B ubiquityl Lys120 antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H2B ubiquityl Lys120 antibody (1 µg/ml).
for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
