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Histone H2BK120ub1 antibody (mAb)

Clone: 56
商品コード: 39623 Format: 100 µg ¥67,000 Buy Now
商品コード: 39624 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:26 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated

for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

Published Applications:
ChIP
WB

for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Immunogen

This Histone H2B ubiquityl Lys120 antibody was raised against a branched peptide corresponding the the ubiquitin conjugation site at Lys120 of human Histone H2B.

for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)References

 
Histone H2BK120ub1 antibody (mAb) tested by Western blot.

Histone H2B ubiquityl Lys120 antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H2B ubiquityl Lys120 antibody (1 µg/ml).

for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Background

Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Histone H2B is ubiquitylated at lysine 120 in vertebrates. H2B ubiquityl Lys120 is found preferentially associated with the transcribed regions of highly expressed genes. It is possible that H2B ubiquitylation is associated with transcriptional elongation.

for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H2B ubiquityl Lys120 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2BK120ub1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H2BK120ub1 antibody (mAb).

for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H2B ubiquityl Lys120 antibody (mAb) (Clone 56)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H2BK120ub1 antibody (mAb)
 

Data Thumbnails

Histone H2BK120ub1 antibody (mAb) tested by Western blot.

Western blot of Histone H2BK120ub1 mAb.
(Click image to enlarge and see details.)