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Antibodies

Uhrf1 antibody (pAb)

Catalog No: 39625 Format: 200 µl $340 Buy Now
Catalog No: 39626 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:Serum
Purification:None
Applications:WB
Reactivity:Human
Molecular Weight:96 kDa

Applications Table

Western Blot Chromatin IP
1:500 - 1:1,000

for Uhrf1 antibody (pAb)Background

Uhrf1 (ubiquitin-like, containing PHD and RING finger domains 1, also known as ICBP90, RNF106 or NP95) is a transcription and cell cycle regulator. It functions as a putative E3 ubiquitin-protein ligase. Uhrf1 is important for G1/S transition of the cell cycle. It is also believed that Uhrf1 may help recruit the DNA methyltransferase, DNMT1, to hemimethylated DNA and assist in the stabilization of DNMT1 with chromatin.

for Uhrf1 antibody (pAb)Immunogen

Raised against a peptide corresponding to the N-terminal half of human Uhrf1.

for Uhrf1 antibody (pAb)Application Notes

This Uhrf1 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution).

for Uhrf1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol, 0.035% sodium azide.

for Uhrf1 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Uhrf1 antibody.

 
Western blot of Uhrf1 pAb

Uhrf1 pAb tested by Western blot. HEK293 nuclear extract (20 µg per lane) was probed with Uhrf1 pAb (1:500).

for Uhrf1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Uhrf1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

Uhrf1 antibody (pAb)
 

Data Thumbnails

Western blot of Uhrf1 pAb

Western blot of Uhrf1 rabbit pAb. (Click image to enlarge and see details.)