SMC1 phospho Ser957 antibody (pAb)

for SMC1 phospho Ser957 antibody (pAb)Background

The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC1 has a myosin-like ATPase domain that serves as a molecular motor to help organize chromatin and is part of the cohesin complex that facilitates chromosome cohesion during the cell cycle. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. SMC1 is also involved in DNA damage repair. Subsequent to double strand DNA breaks, SMC1 is phosphorylated by the ATM kinase. Phosphorylated SMC1 is crucial to the successful repair of DNA damage. Defects in SMC1 isoform A are the cause of Cornelia de Lange syndrome type 2, an inherited developmental disorder associated with malformations affecting multiple systems.

for SMC1 phospho Ser957 antibody (pAb)Immunogen

Peptide containing phospho-serine 957 of human SMC1.

for SMC1 phospho Ser957 antibody (pAb)Application Notes

This SMC1 phospho Ser957 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution).

for SMC1 phospho Ser957 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.

for SMC1 phospho Ser957 antibody (pAb)Positive Control

Nuclear extract of HeLa cells treated with etoposide (100 µM for 2 hours) can be used as positive control for SMC1 phospho Ser957 antibody.

for SMC1 phospho Ser957 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for SMC1 phospho Ser957 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.