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Histone H3K4me1 antibody (mAb)
| RRID: AB_2793284 |
| Clone: MABI 0302 |
| 商品コード: 39635 | Format: 100 µg | ¥92,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP-Seq
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
The Histone H3 monomethyl Lys4 antibody was raised against a peptide containing monomethyl lysine 4 of human histone H3.
Buffer
PBS pH 7.5 containing 30% glycerol. 0.3M NaCl, and 0.035% sodium azide. Sodium azide is highly toxic.
Histone H3K4me1 antibody (mAb) tested by ChIP-Seq
ChIP was performed using 10 µg of chromatin from MCF7 cells and 4 µg of antibody. ChIP DNA was sequenced and 19 million sequence tags were mapped to identify Histone H3K4me1 binding sites.
Histone H3 monomethyl Lys4 antibody (mAb) tested by Western blot.
HeLa acid extract probed with Histone H3 monomethyl Lys4 antibody (mAb) (2 µg/ml dilution).
Histone H3 monomethyl Lys4 antibody (mAb) tested by Dot Blot
Dot Blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys4 antibody (mAb) for monomethyl Lys4 histone H3. Methylated peptides corresponding to the immunogen or related peptides were spotted onto PVDF and probed with antibody at 1 µg/ml. Lane 1: unmodified peptide, Lane 2: Monomethyl Lys4, Lanes 3-8: various negative control peptides.
Histone H3 monomethyl Lys4 antibody (mAb) tested by CUT&Tag
CUT&Tag was performed using 100,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 5 million reads, and H3K4me1 data is shown for Chromosome 7.
qPCR Primers
Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4me1 antibody (mAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
This antibody is manufactured by MAB Institute, Inc.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
