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Histone H3.3S31ph antibody (pAb)
| RRID: AB_2793286 |
| 商品コード: 39637 | Format: 100 µl | ¥66,800 | Buy |
| 商品コード: 39638 | Format: 10 µl | ¥18,800 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
WB
IF
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3.3 phospho Ser31 antibody was raised against a peptide containing phospho Ser31 of Histone H3.3.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
View our Guide to Histone Modifications and Biological Function.
Histone H3.3 phospho Ser31 antibody tested by Western blot.
Detection of Histone H3.3 by Western blot. The analysis was performed using 20 µg of untreated (lane 1) or colcemid treated (lane 2) HeLa acid extract with a 1:1000 dilution of Histone H3.3 phospho Ser31 antibody.
Histone H3.3 phospho Ser31 antibody tested by Dot blot.
Dot blot analysis was used to confirm the specificity of Histone H3.3 phospho Ser31 antibody. Peptides corresponding to histone H3.3 and regions around major sites of histone H3 phosphorylation (serine 10 and serine 28) were spotted onto PVDF and probed with 39637 at a dilution of 1:10,000. The amount of peptide (in picomoles) spotted is indicated next to each row.
Lane 1: peptide phosphorylated at serine 31 of H3.3.
Lane 2: unmodified serine 31 peptide.
Lane 3: peptide phosphorylated at serine 10 of H3.
Lane 4: peptide phosphorylated at serine 28 of H3.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
