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Pht1 / H2AZ acetyl antibody (pAb)
| Catalog No: 39642 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39643 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for Pht1 / H2AZ acetyl antibody (pAb)Background
Pht1 is the S. pombe homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture. It is also implicated in suppressing the accumulation of anti-sense transcripts through interaction with RNAi machinery and heterochromatin proteins.
for Pht1 / H2AZ acetyl antibody (pAb)Immunogen
This Pht1 / H2AZ acetyl antibody was raised against a peptide containing acetyl-lysine at positions 5, 7, 12 and 16 from the amino terminus of Pht1, the histone H2AZ variant in S. pombe.
for Pht1 / H2AZ acetyl antibody (pAb)Application Notes
This Pht1 / H2AZ acetyl antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution).
for Pht1 / H2AZ acetyl antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.
for Pht1 / H2AZ acetyl antibody (pAb)Positive Control
Whole-cell extracts from S. pombe can be used as a positive control for Pht1 / H2AZ acetyl antibody.
Pht1 / H2AZ acetyl pAb tested by Western blot.
S. pombe whole-cell extract probed with Pht1 / H2AZ acetyl pAb (1:500 dilution).
Pht1 / H2AZ acetyl pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Pht1 / H2AZ acetyl pAb for acetylation of Pht1, the S.pombe variant for Histone H2AZ. Modified and unmodified peptides were spotted onto PVDF and probed with Pht1 / H2AZ acetyl pAb at a 1:1,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row.
Lane 1: Peptide of acetylated Pht1
Lane 2: Unmodified Pht1 peptide
for Pht1 / H2AZ acetyl antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Pht1 / H2AZ acetyl antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
