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Pht1 / Histone H2A.Zac (pan-acetyl) antibody (pAb)
| RRID: AB_2793288 |
| 商品コード: 39642 | Format: 100 µl | ¥66,800 | Buy |
| 商品コード: 39643 | Format: 10 µl | ¥18,800 | Buy |
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Applications
Immunogen
This Pht1 / H2AZ acetyl antibody was raised against a peptide containing acetyl-lysine at positions 5, 7, 12 and 16 from the amino terminus of Pht1, the histone H2AZ variant in S. pombe.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
View our Guide to Histone Modifications and Biological Function.
Pht1 / H2AZ acetyl pAb tested by Western blot.
S. pombe whole-cell extract probed with Pht1 / H2AZ acetyl pAb (1:500 dilution).
Pht1 / H2AZ acetyl pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Pht1 / H2AZ acetyl pAb for acetylation of Pht1, the S.pombe variant for Histone H2AZ. Modified and unmodified peptides were spotted onto PVDF and probed with Pht1 / H2AZ acetyl pAb at a 1:1,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Peptide of acetylated Pht1. Lane 2: Unmodified Pht1 peptide.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
