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Antibodies

Htz1 antibody (pAb)

Catalog No: 39647 Format: 100 µl $340 Buy Now
Catalog No: 39648 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:ChIP, WB
Reactivity:Budding Yeast
Molecular Weight:15 kDa

Applications Table

Western Blot Chromatin IP
1:500 - 1:1,000 2 - 5 µl per ChIP

for Htz1 antibody (pAb)Background

Htz1 is the S. cerevesiae homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture.

for Htz1 antibody (pAb)Immunogen

This antibody was raised against full-length Htz1 protein from S. cerevisiae.

for Htz1 antibody (pAb)Application Notes

This Htz1 antibody has been validated for use in chromatin IP (2 - 5 µl per ChIP) and Western blotting (1:500 - 1:1,000 dilution).

for Htz1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.

for Htz1 antibody (pAb)Positive Control

Whole-cell extracts from S. pombe can be used as a positive control for Htz1 antibody.

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Htz1 antibody (pAb).

 
Htz1 pAb tested by Western blot.

Htz1 pAb tested by Western blot.
   Lane 1: S. cerevesiae whole-cell extract probed with Htz1 pAb at a 1:500 dilution.
   Lane 2: Extract from a wild-type strain probed with Htz1 pAb at a 1:1000 dilution.
   Lane 3: Extract from a strain deleted for the Htz1 gene probed with Htz1 pAb at a 1:1000 dilution.

<b>Htz1 pAb tested by ChIP.</b><br>Chromatin immunoprecipitation performed on <i>S. cerevesiae chromatin</i> using 2 µl of Htz1 pAb. Real-time quantitative PCR was performed using primers corresponding to the Act1 gene open reading frame and the GAL1 gene TATA box.

Htz1 pAb tested by ChIP.
Chromatin immunoprecipitation performed on S. cerevesiae chromatin using 2 µl of Htz1 pAb. Real-time quantitative PCR was performed using primers corresponding to the Act1 gene open reading frame and the GAL1 gene TATA box.

for Htz1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Htz1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

Htz1 antibody (pAb)
 

Data Thumbnails

Htz1 pAb tested by Western blot.

Western blot of Htz1 pAb.
(Click image to enlarge and see details.)

<b>Htz1 pAb tested by ChIP.</b><br>Chromatin immunoprecipitation performed on <i>S. cerevesiae chromatin</i> using 2 µl of Htz1 pAb. Real-time quantitative PCR was performed using primers corresponding to the Act1 gene open reading frame and the GAL1 gene TATA box.

ChIP of Histone Htz1 pAb.
(Click image to enlarge and see details.)