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Htz1 / Histone H2A.Z antibody (pAb)

商品コード: 39647 Format: 100 µl ¥67,000 Buy Now
商品コード: 39648 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:15 kDa
Reactivity:Budding Yeast

Applications

ChIP Validated Western Blot Validated

for Htz1 / Histone H2A.Z antibody (pAb) (H2AZ)Application Notes


Validated Applications:
ChIP: 2 - 5 µl per ChIP
WB: 1:500 - 1:1,000 dilution

Published Applications:

WB

for Htz1 / Histone H2A.Z antibody (pAb) (H2AZ)Immunogen

This Htz1 antibody was raised against full-length Htz1 protein from S. cerevisiae.

for Htz1 / Histone H2A.Z antibody (pAb) (H2AZ)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Htz1 / Histone H2A.Z antibody (pAb) (H2AZ)References

 
Htz1 / Histone H2A.Z antibody (pAb) tested by ChIP.

Htz1 pAb tested by ChIP.
Chromatin immunoprecipitation performed on S. cerevesiae chromatin using 2 µl of Htz1 pAb. Real-time quantitative PCR was performed using primers corresponding to the Act1 gene open reading frame and the GAL1 gene TATA box.

Htz1 / Histone H2A.Z antibody (pAb) tested by Western blot.

Htz1 pAb tested by Western blot.
   Lane 1: S. cerevesiae whole-cell extract probed with Htz1 pAb at a 1:500 dilution.
   Lane 2: Extract from a wild-type strain probed with Htz1 pAb at a 1:1000 dilution.
   Lane 3: Extract from a strain deleted for the Htz1 gene probed with Htz1 pAb at a 1:1000 dilution.

for Htz1 / Histone H2A.Z antibody (pAb) (H2AZ)Background

Htz1 is the S. cerevesiae homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture.

for Htz1 / Histone H2A.Z antibody (pAb) (H2AZ)Positive Control

Whole-cell extracts from S. pombe can be used as a positive control for Htz1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Htz1 / Histone H2A.Z antibody (pAb).

for Htz1 / Histone H2A.Z antibody (pAb) (H2AZ)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Htz1 / Histone H2A.Z antibody (pAb) (H2AZ)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Htz1 / Histone H2A.Z antibody (pAb)
 

Data Thumbnails

Htz1 / Histone H2A.Z antibody (pAb) tested by ChIP.

ChIP of Htz1 / Histone H2A.Z pAb.
(Click image to enlarge and see details.)

Htz1 / Histone H2A.Z antibody (pAb) tested by Western blot.

Western blot of Htz1 / Histone H2A.Z pAb.
(Click image to enlarge and see details.)