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Histone H3R8me2a (asymmetric) antibody (pAb)

商品コード: 39651 Format: 100 µl ¥69,000 Buy Now
商品コード: 39652 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Dot Blot Validated

for Histone H3R8me2a (asymmetric) antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
ChIP-Seq: 10 µl each
WB: 1:500 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

Published Applications:
ChIP
WB

for Histone H3R8me2a (asymmetric) antibody (pAb)Immunogen

This Histone H3 dimethyl Arg8 asymmetric antibody was raised against a peptide containing dimethyl-Arg8 (asymmetric) of human histone H3.

for Histone H3R8me2a (asymmetric) antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3R8me2a (asymmetric) antibody (pAb)References

 
Histone H3R8me2a (asymmetric) antibody (pAb) tested by ChIP-Seq.

Histone H3R8me2a antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from a mouse osteoblast cell line and 7 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3R8me2a binding sites. The image shows binding across a region of chromosome 5. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

Histone H3R8me2a (asymmetric) antibody (pAb) tested by Western blot.

Histone H3 dimethyl Arg8 asymmetric pAb tested by Western blot.
Nuclear extract from U2OS cells (20 µg) or HEK293 cells (20 µg) was probed with Histone H3 dimethyl Arg8 asymmetric pAb (1:500 dilution).

Histone H3R8me2a (asymmetric) antibody (pAb) tested by dot blot analysis.

Histone H3 dimethyl Arg8 asymmetric pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg8 asymmetric pAb for dimethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg8 asymmetric pAb at 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Top Panel: Lane 1: unmod Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (sym). Lane 4: dimethyl-Arg2 H3 (asym). Lane 5: unmod Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (sym). Lane 8: dimethyl-Arg8 H3 (asym). Bottom Panel: Lane 1: unmod Arg17 H3. Lane 2: monomethyl-Arg17. Lane 3: dimethyl-Arg17 H3 (sym). Lane 4: dimethyl-Arg17 H3 (asym). Lane 5: unmod Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (sym). Lane 8: dimethyl-Arg26 H3 (asymmetric). No detection of peptides (unmodified, mono-, di-, or tri-methylated) corresponding to Lys4, Lys9, Lys18, Lys23 and Lys27 of histone H3 was observed.

for Histone H3R8me2a (asymmetric) antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.

for Histone H3R8me2a (asymmetric) antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Histone H3R8me2a (asymmetric) antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3R8me2a (asymmetric) antibody (pAb).

for Histone H3R8me2a (asymmetric) antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3R8me2a (asymmetric) antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3R8me2a (asymmetric) antibody (pAb)
 

Data Thumbnails

Histone H3R8me2a (asymmetric) antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Histone H3R8me2a (asymmetric) pAb.
(Click image to enlarge and see details.)

Histone H3R8me2a (asymmetric) antibody (pAb) tested by Western blot.

Western blot of Histone H3R8me2a (asymmetric) pAb.
(Click image to enlarge and see details.)

Histone H3R8me2a (asymmetric) antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3R8me2a (asymmetric) pAb.
(Click image to enlarge and see details.)