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Histone H3 dimethyl Lys23 antibody (pAb)
| Catalog No: 39653 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39654 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for Histone H3 dimethyl Lys23 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.
for Histone H3 dimethyl Lys23 antibody (pAb)Immunogen
This antibody was raised against a peptide containing dimethyl-Lys23 of human histone H3.
for Histone H3 dimethyl Lys23 antibody (pAb)Application Notes
This antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution).
for Histone H3 dimethyl Lys23 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
Histone H3 dimethyl Lys23 pAb tested by Western blot.
HEK293 nuclear extract (25 µg) was probed with Histone Histone H3 dimethyl Lys23 pAb (1:500 dilution).
Histone H3 dimethyl Lys23 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys23 pAb for dimethyl-Lys23 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with Histone H3 dimethyl Lys23 pAb at a dilution of 1: 2,000. The amount of peptide (in picomoles) spotted is indicated next to each row.
Top Panel:
Lane 1: dimethyl Lys14 peptide.
Lane 2: monomethyl Lys18 peptide.
Lane 3: dimethyl Lys18 peptide.
Lane 4: trimethyl Lys18 peptide.
Lane 5: unmodified Lys23 peptide.
Lane 6: monomethyl Lys23 peptide.
Lane 7: dimethyl Lys23 peptide.
Lane 8: trimethyl Lys23 peptide.
Lane 9: unmodified Lys27 peptide.
Lane 10: monomethyl Lys27 peptide.
Lane 11: dimethyl Lys27 peptide.
Lane 12: trimethyl Lys27 peptide.
Bottom Panel:
Lane 1: unmodified Lys4 peptide.
Lane 2: monomethyl Lys4 peptide.
Lane 3: dimethyl Lys4 peptide.
Lane 4: trimethyl Lys4 peptide.
Lane 5: unmodified Lys9 peptide.
Lane 6: monomethyl Lys9 peptide.
Lane 7: dimethyl Lys9 peptide.
Lane 8: trimethyl Lys9 peptide.
for Histone H3 dimethyl Lys23 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 dimethyl Lys23 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
