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MAD1 antibody (mAb)

Clone: 9B10
Aliases: MXD1, MAD, BHLHC58
商品コード: 39659 Format: 100 µg ¥67,000 Buy Now
商品コード: 39660 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:85 kDa
Reactivity:Human, Mouse

Applications

Immunofluorescence Validated Immunocytochemistry Validated

for MAD1 antibody (mAb) (Clone 9B10)Application Notes


Published Applications:
ICC/IF
See references for more information. Individual optimization may be required.

for MAD1 antibody (mAb) (Clone 9B10)Immunogen

This MAD1 antibody was raised against a peptide corresponding to amino acids 331-718 of mouse MAD1.

for MAD1 antibody (mAb) (Clone 9B10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MAD1 antibody (mAb) (Clone 9B10)References

for MAD1 antibody (mAb) (Clone 9B10)Background

MAD1 (MAD1L1, Mitotic arrest deficient 1) is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD1 homoderizes with MAD2L1 and is present at the kinetochore. Defects in MAD1 are involved in the development and/or progression of various types of cancer.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with MAD1 antibody (mAb).

for MAD1 antibody (mAb) (Clone 9B10)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MAD1 antibody (mAb) (Clone 9B10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MAD1 antibody (mAb)