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Histone H4 trimethyl Lys20 antibody (mAb)
| Clone: 6F8-D9 |
| Catalog No: 39671 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39672 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | 5 - 10 µg per ChIP |
for Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9)Background
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.
for Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9)Immunogen
This Histone H4 trimethyl Lys20 antibody (mAb) was raised against a peptide containing trimethyl Lys20 of Histone H4.
for Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9)Application Notes
This Histone H4 trimethyl Lys20 antibody (mAb) has been validated for use in Western blotting (1 - 2 µg/ml dilution) and ChIP (5 - 10 µg per ChIP).
Slight reactivity towards histone H4 dimethyl Lys20 might be observed under certain conditions.
for Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9)Buffer
Purified mouse IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H4 trimethyl Lys20 antibody (mAb).
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4 trimethyl Lys20 antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H4 trimethyl Lys20 antibody (mAb).
Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) (2 µg/ml dilution).
Histone H4 trimethyl Lys20 mAb (Clone 6F8-D9) tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa chromatin (1.5 x 10^6 cell equivalents per ChIP) using 5 µg of Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for human SAT-alpha repeats. Data are presented as Fold Enrichment of the ChIP antibody signal compared to the negative control IgG (which has been normalized to 1.0) using the ddCT method.
Histone H4 trimethyl Lys20 mAb (Clone 6F8-D9) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) for trimethyl-Lys20 of histone H4. Modified and unmodified peptides were spotted onto PVDF and probed with Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) at a dilution of 2 µg/ml. The amount of peptide (in picomoles) spotted is indicated next to each row.
Lane 1: monomethyl Lys20 peptide.
Lane 2: dimethyl Lys20 peptide.
Lane 3: trimethyl Lys20 peptide.
Lane 4: Unmodified Lys20 peptide.
for Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
