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Mi-2 beta antibody (mAb)

Clone: 3F2/4
Aliases: CHD4, Mi-2b
商品コード: 39695 Format: 100 µg ¥67,000 Buy Now
商品コード: 39696 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:240 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for Mi-2 beta antibody (mAb) (Clone 3F2/4)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

for Mi-2 beta antibody (mAb) (Clone 3F2/4)Immunogen

This Mi-2 beta antibody was raised against a synthetic peptide derived from human Mi-2B.

for Mi-2 beta antibody (mAb) (Clone 3F2/4)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Mi-2 beta antibody (mAb) (Clone 3F2/4)References

 
Mi-2 beta antibody (mAb) tested by Western blot.

Mi-2 beta pAb tested by Western blot.
Nuclear extract of Jurkat cells stained with Mi-2 beta mAb at a dilution of 2 µg/ml.

for Mi-2 beta antibody (mAb) (Clone 3F2/4)Background

Mi-2β (Mi-2beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).

for Mi-2 beta antibody (mAb) (Clone 3F2/4)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for Mi-2 beta antibody.

for Mi-2 beta antibody (mAb) (Clone 3F2/4)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Mi-2 beta antibody (mAb) (Clone 3F2/4)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Mi-2 beta antibody (mAb)
 

Data Thumbnails

Mi-2 beta antibody (mAb) tested by Western blot.

Western blot of Mi-2 beta mAb.
(Click image to enlarge and see details.)