Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

Histone H3 dimethyl Arg2 symmetric antibody (pAb)

Catalog No: 39703 Format: 100 µl $340 Buy Now
Catalog No: 39704 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:DB, WB
Reactivity:Human, Other (Wide Range)
Molecular Weight:17 kDa

Applications Table

Western Blot Chromatin IP
1:500

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine.

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Immunogen

This Histone H3 dimethyl Arg2 (H3R2me2s) antibody was raised against a peptide containing dimethyl-Arg2 (symmetric) of human histone H3.

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Application Notes

This Histone H3 dimethyl Arg2 symmetric (H3R2me2s) antibody has been validated for use in Western blotting (1:500 dilution).

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 dimethyl Arg2 symmetric pAb.

 
Histone H3 dimethyl Arg2 symmetric pAb tested by Western blot.

Histone H3 dimethyl Arg2 symmetric pAb tested by Western blot.
Western blot performed using Histone H3 dimethyl Arg2 symmetric pAb at a dilution of 1:500. Lane 1: Nuclear extract from HEK293 cells (25 µg). Lane 2: Nuclear extract from HeLa cells (20 µg).

Histone H3 dimethyl Arg2 symmetric pAb tested by dot blot.

Histone H3 dimethyl Arg2 symmetric pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg2 symmetric pAb for dimethyl-arginine 2 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg2 symmetric pAb at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row.

Top Panel:
Lane 1: unmodified Arg2 peptide.
Lane 2: monomethyl-Arg2 H3 peptide.
Lane 3: dimethyl-Arg2 H3 (symmetric) peptide.
Lane 4: dimethyl-Arg2 H3 (asymmetric) peptide.
Lane 5: unmodified Arg8 peptide.
Lane 6: monomethyl-Arg8 H3 peptide.
Lane 7: dimethyl-Arg8 H3 (symmetric) peptide.
Lane 8: dimethyl-Arg8 H3 (asymmetric) peptide.

Bottom Panel:
Lane 1: unmodified Arg17 H3 peptide.
Lane 2: monomethyl-Arg17 peptide.
Lane 3: dimethyl-Arg17 H3 (symmetric) peptide.
Lane 4: dimethyl-Arg17 H3 (asymmetric) peptide.
Lane 5: unmodified Arg26 peptide.
Lane 6: monomethyl-Arg26 H3 peptide.
Lane 7: dimethyl-Arg26 H3 (symmetric) peptide.
Lane 8: dimethyl-Arg26 H3 (asymmetric) peptide.

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting

Technical Data Sheet

Histone H3 dimethyl Arg2 symmetric antibody (pAb)
 

Data Thumbnails

Histone H3 dimethyl Arg2 symmetric pAb tested by Western blot.

Western blot of Histone H3 dimethyl Arg2 symmetric pAb.
(Click image to enlarge and see details).

Histone H3 dimethyl Arg2 symmetric pAb tested by dot blot.

Dot blot of Histone H3 dimethyl Arg2 symmetric pAb.
(Click image to enlarge and see details).