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Histone H3R2me2s (symmetric) antibody (pAb)

商品コード: 39703 Format: 100 µl ¥67,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 dilution

Published Applications:
IF

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Immunogen

This Histone H3 dimethyl Arg2 (H3R2me2s) antibody was raised against a peptide containing dimethyl-Arg2 (symmetric) of human histone H3.

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Buffer

Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)References

 
Histone H3R2me2s (symmetric) antibody (pAb) tested by Western blot.

Histone H3 dimethyl Arg2 symmetric pAb tested by Western blot.
Western blot performed using Histone H3 dimethyl Arg2 symmetric pAb at a dilution of 1:500. Lane 1: Nuclear extract from HEK293 cells (25 µg). Lane 2: Nuclear extract from HeLa cells (20 µg).

Histone H3R2me2s (symmetric) antibody (pAb) tested by dot blot analysis.

Histone H3 dimethyl Arg2 symmetric pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg2 symmetric pAb for dimethyl-arginine 2 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg2 symmetric pAb at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Top Panel: Lane 1: unmodified Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (symmetric). Lane 4: dimethyl-Arg2 H3 (asymmetric). Lane 5: unmodified Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (symmetric). Lane 8: dimethyl-Arg8 H3 (asymmetric).Bottom Panel: Lane 1: unmodified Arg17 H3. Lane 2: monomethyl-Arg17. Lane 3: dimethyl-Arg17 H3 (symmetric). Lane 4: dimethyl-Arg17 H3 (asymmetric). Lane 5: unmodified Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (symmetric). Lane 8: dimethyl-Arg26 H3 (asymmetric).

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine.

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 dimethyl Arg2 symmetric pAb.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3R2me2s (symmetric) antibody (pAb).

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 dimethyl Arg2 symmetric antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3R2me2s (symmetric) antibody (pAb)
 

Data Thumbnails

Histone H3R2me2s (symmetric) antibody (pAb) tested by Western blot.

Western blot of Histone H3R2me2s (symmetric) pAb.
(Click image to enlarge and see details.)

Histone H3R2me2s (symmetric) antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3R2me2s (symmetric) pAb.
(Click image to enlarge and see details.)