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Histone H4 dimethyl Arg3, asymmetric antibody (pAb)
| Catalog No: 39705 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39706 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:500 - 1:2,000 | — | 1:1,000 |
for Histone H4 dimethyl Arg3, asymmetric antibody (pAb)Background
Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine.
Asymmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional activation by nuclear hormone receptors. Arg3 methylation has been reported to facilitate the subsequent acetylation of histone H4 by the acetyltransferase p300. Histone H4 dimethyl Arg3 asymmetric methylation (H4R2me2a) is catalyzed by PRMT1 and PRMT6.
for Histone H4 dimethyl Arg3, asymmetric antibody (pAb)Immunogen
This Histone H4 dimethyl Arg3, asymmetric antibody was raised against a synthetic peptide containing dimethyl-Arg3 (asymmetric) of human histone H4.
for Histone H4 dimethyl Arg3, asymmetric antibody (pAb)Application Notes
This Histone H4 dimethyl Arg3 asymmetric antibody was validated in Western blotting (1:500 - 1:2,000) and immunofluorescence (1:1,000).
To avoid possible cross-reactivity to other methylated proteins, use acid extraction of cells for Western blotting.
for Histone H4 dimethyl Arg3, asymmetric antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.
for Histone H4 dimethyl Arg3, asymmetric antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H4 dimethyl Arg3, asymmetric antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4 dimethyl Arg3, asymmetric antibody (pAb).
Histone H4 dimethyl Arg3 asymmetric antibody tested by Western blot.
Acid extract of HeLa cells (10 µg) tested by Western blot using Histone H4 dimethyl Arg3 asymmetric antibody at a dilution of 1:1,000.
Histone H4 dimethyl Arg3 asymmetric antibody (pAb) tested by immunofluorescence.
HeLa cells stained with Histone H4 dimethyl Arg3 asymmetric pAb (1:1,000). Top: Histone H4 dimethyl Arg3 asymmetric pAb. Middle: DAPI. Bottom: merge.
Histone H4 dimethyl Arg3 asymmetric antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 asymmetric antibody for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 asymmetric antibody at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified H4 peptide.
Lane 2: monomethyl-Arg3 H4, linear peptide.
Lane 3: dimethyl-Arg3 H4 (asymmetric) peptide.
Lane 4: dimethyl-Arg3 H4 (symmetric) peptide.
Histone H4 dimethyl Arg3, asymmetric antibody specificity tested by peptide array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4 dimethyl Arg3, asymmetric antibody was applied at a dilution of 1:20,000 to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. Recognition of the H4 dimethyl Arg3, asymmetric peptides by the antibody is blocked by phosphorylation at Ser1.
Array Data File
for Histone H4 dimethyl Arg3, asymmetric antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H4 dimethyl Arg3, asymmetric antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
