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Histone H4R3me2a (asymmetric) antibody (pAb)
| RRID: AB_2793313 |
| 商品コード: 39705 | Format: 100 µl | ¥92,000 | Buy |
| 商品コード: 39006 | Format: 50 µl | ¥55,000 | Buy |
| 商品コード: 39706 | Format: 10 µl | ¥25,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ChIP
WB
IF
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H4 dimethyl Arg3, asymmetric antibody was raised against a synthetic peptide containing dimethyl-Arg3 (asymmetric) of human histone H4.
Buffer
Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Histone H4R3me2a (asymmetric) antibody (pAb)tested by Western blot.
Nuclear extract of HeLa cells (20 µg) tested by Western blot using Histone H4R3me2a (asymmetric) antibody (pAb) at a dilution of 1:1000.
Histone H4R3me2a (asymmetric) antibody (pAb)tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 asymmetric antibody for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 asymmetric antibody at 1:2500. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: H4R3me unmodified.
Lane 2: H4R3me2a peptide.
Lane 3: H4R3me2s peptide.
Lane 4: H4R3me1 peptide.
Histone H4R3me2a (asymmetric) antibody (pAb) specificity tested by peptide array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4R3me2a antibody was applied at a dilution of 1:20,000 to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 16 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4R3me2a (asymmetric) antibody (pAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
