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Histone H1 antibody (pAb)

商品コード: 39707 Format: 100 µl ¥67,000 Buy Now
商品コード: 39708 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:31 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Histone H1 antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:500 - 1:5,000 dilution

Published Applications:
WB

for Histone H1 antibody (pAb)Immunogen

This Histone H1 antibody was raised against calf thymus histone H1.

for Histone H1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 61201) of this antibody that was purified by Protein A Chromatography is also available.

for Histone H1 antibody (pAb)References

 
Histone H1 antibody (pAb) tested by immunofluorescence.

Histone H1 antibody tested by immunofluorescence.
Detection of Histone H1 by immunofluorescence. HeLa cells were stained with Histone H1 antibody at a dilution of 1:1,000. Top panel: Histone H1 antibody staining. Middle panel: DAPI. Bottom panel: merge.

Histone H1 antibody (pAb) tested by Western blot.

Histone H1 pAb tested by Western blot.
HeLa nuclear extract (20 µg) probed with Histone H1 pAb (1:2,000 dilution).

for Histone H1 antibody (pAb)Background

The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression.

for Histone H1 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H1 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H1 antibody (pAb).

for Histone H1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H1 antibody (pAb)
 

Data Thumbnails

Histone H1 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H1 pAb.
(Click image to enlarge and see details.)

Histone H1 antibody (pAb) tested by Western blot.

Western blot of Histone H1 pAb.
(Click image to enlarge and see details.)