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Histone H1 antibody (pAb)
| Catalog No: 39707 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39708 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:1,000 - 1:5,000 | — | 1:1,000 |
for Histone H1 antibody (pAb)Background
The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression.
for Histone H1 antibody (pAb)Immunogen
This antibody was raised against calf thymus histone H1.
for Histone H1 antibody (pAb)Application Notes
This antibody has been validated for use in Western blotting (1:1,000 - 1:5,000 dilution) and immunofluorescence (1:1,000).
for Histone H1 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for Histone H1 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H1 antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H1 antibody (pAb).
Histone H1 pAb tested by Western blot.
HeLa nuclear extract (20 µg) probed with Histone H1 pAb (1:2,000 dilution).
Histone H1 antibody tested by immunofluorescence.
Detection of Histone H1 by immunofluorescence. HeLa cells were stained with Histone H1 antibody at a dilution of 1:1,000. Top panel: Histone H1 antibody staining. Middle panel: DAPI. Bottom panel: merge.
for Histone H1 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H1 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
