Histone H3R17me2a (asymmetric) antibody (pAb)

RRID: AB_2793315
商品コード: 39709 Format: 100 µl ¥92,000 Buy
商品コード: 39710 Format: 10 µl ¥18,800 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunohistochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP
WB
IHC(P)
IF

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H3 dimethyl Arg17 asymmetric antibody was raised against a peptide containing dimethyl-Arg17 (asymmetric) of human histone H3.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 
Histone H3R17me2a (asymmetric) antibody (pAb) tested by Western blot.

Histone H3 dimethyl Arg17 asymmetric antibody (pAb) tested by Western blot.
HeLa nuclear extract (20 µg) probed with Histone H3 dimethyl Arg17 asymmetric antibody at a 1:500 dilution.

Histone H3R17me2a (asymmetric) antibody (pAb) tested by dot blot analysis.

Histone H3 dimethyl Arg17 asymmetric pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg17 asymmetric pAb for dimethyl Arg17 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg17 asymmetric pAb at a dilution of 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Top panel Lane 1: unmodified Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (sym). Lane 4: dimethyl-Arg2 H3 (asym). Lane 5: unmodified Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (sym). Lane 8: dimethyl-Arg8 H3 (asym).
Bottom panel Lane 1: unmodified Arg17. Lane 2: monomethyl-Arg17 H3. Lane 3: dimethyl-Arg17 H3 (asym). Lane 4: dimethyl-Arg17 H3 (sym). Lane 5: unmodified Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (asym). Lane 8: dimethyl-Arg26 H3 (sym).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3R17me2a (asymmetric) antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting