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HDAC3 antibody (mAb)

Clone: 3G6
Aliases: HD3, RPD3, RPD3-2
商品コード: 39717 Format: 100 µg ¥67,000 Buy Now
商品コード: 39718 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:50 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for HDAC3 antibody (mAb) (Clone 3G6)Application Notes


Published Applications:
IP
WB
See references for more information. Individual optimization may be required.

This antibody can be used to immunoprecipitate HDAC3 activity as well as the protein itself
See references for more information. Individual optimization may be required.

for HDAC3 antibody (mAb) (Clone 3G6)Immunogen

This HDAC3 antibody was raised against a synthetic peptide corresponding to amino acids 411-428 of human HDAC3.

for HDAC3 antibody (mAb) (Clone 3G6)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for HDAC3 antibody (mAb) (Clone 3G6)References

for HDAC3 antibody (mAb) (Clone 3G6)Background

HDAC3 (Histone Deacetylase 3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

By forming multi-protein complexes with the co-repressors SMRT and N-CoR, HDAC3 regulates the transcription of a plethora of genes. A growing list of non-histone proteins extends the role of HDAC3 beyond transcriptional repression. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

for HDAC3 antibody (mAb) (Clone 3G6)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HDAC3 mAb (Clone 3G6).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use HDAC3 antibody (mAb).

for HDAC3 antibody (mAb) (Clone 3G6)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HDAC3 antibody (mAb) (Clone 3G6)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HDAC3 antibody (mAb)