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CID antibody (pAb)
| Catalog No: 39719 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39720 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| Not recommended. WB-validated version available: 39713. |
— | 1:500 |
for CID antibody (pAb)Background
CID (Centromer Identifier) is the Drosophila histone H3 variant which replaces canonical H3 in centromeric chromatin at the inner plate of the kinetochore (similar to mammalian CENP-A). CID is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. It may serve as an epigenetic mark that propagates centromere identity through DNA replication and cell division.
for CID antibody (pAb)Immunogen
This antibody was raised against recombinant CID protein from Drosophila melanogaster.
for CID antibody (pAb)Application Notes
This antibody has been validated for use in immunofluorescence (1:500 dilution). For an antibody against CID that works in Western blotting, use Catalog No. 39713.
for CID antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CID antibody (pAb).
CID pAb tested by immunofluorescence.
DrosophilaSchneider L2 cells stained with CID pAb (1:500 dilution).
for CID antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for CID antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
