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Acf1 antibody (pAb)

Aliases: Acf, ACF, Acf-1, ACF/CHRAC, ACF1, CAF, CG1966, CHRAC, CHRAC-175, dACF, dCHRAC, Dmel\CG1966, p170/p185
商品コード: 39721 Format: 100 µl ¥67,000 Buy Now
商品コード: 39722 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:185 kDa
Reactivity:Drosophila

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Acf1 antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for Acf1 antibody (pAb)Immunogen

This Acf1 antibody was raised against recombinant C-terminal fragment of Acf1 from Drosophila melanogaster.

for Acf1 antibody (pAb)Buffer

PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol. Sodium azide is highly toxic.

for Acf1 antibody (pAb)References

 
Acf1 antibody (pAb) tested by immunofluorescence.

Acf1 pAb tested by immunofluorescence.
Drosophila polytene chromosomes stained with Acf1 pAb (1:1,000 dilution).

Acf1 antibody (pAb) tested by Western blot.

Acf1 pAb tested by Western blot.
Drosophila Schneider L2 cells nuclear extract (10 µg) probed with Acf1 pAb (1:500 dilution).

for Acf1 antibody (pAb)Background

Drosophila Acf1 is a component of ACF, the ATP-utilizing chromatin assembly and remodeling factor protein complex. ACF catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro. The catalytic subunit of ACF is the ISWI ATP-dependant chromatin remodeling protein. ACF is related to CHRAC (Chromatin Accessibility Complex), which also contains Acf1 and ISWI. ACF is involved in the establishment and possibly maintenance of silenced heterochromatic domains in the genome, both at pericentric heterochromatin and at genes repressed by the Polycomb complex.

for Acf1 antibody (pAb)Positive Control

Active Motif's Schneider's Drosophila L2 nuclear extract (Catalog No. 36087) can be used as a positive control for Acf1 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Acf1 antibody (pAb).

for Acf1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Acf1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Acf1 antibody (pAb)
 

Data Thumbnails

Acf1 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Acf1 pAb.
(Click image to enlarge and see details.)

Acf1 antibody (pAb) tested by Western blot.

Western blot of Acf1 pAb.
(Click image to enlarge and see details.)