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Antibodies

HIPK1 antibody (mAb)

Catalog No: 39725 Format: 100 µg $340 Buy Now
Catalog No: 39726 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG
Purification:Protein G Chromatography
Applications:IF
Reactivity:Mouse
Molecular Weight:131 kDa

Applications Table

Western Blot Chromatin IP Immunofluorescence
Published.
See Application Notes below.

for HIPK1 antibody (mAb)Background

HIPK1 (Homeodomain Interacting Protein Kinase 1) is a serine/threonine kinase present in the nucleus that interacts with homeodomain transcription factors and acts as a co-repressor of transcription. It phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. HIPK1 may be involved in malignant squamous cell tumor formation.

for HIPK1 antibody (mAb)Immunogen

This antibody was raised against a recombinant protein including amino acids 702-925 of mouse HIPK1.

for HIPK1 antibody (mAb)Application Notes

This HIPK antibody has been validated for use in immunofluorescence. See references for more details.

for HIPK1 antibody (mAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HIPK1 antibody (mAb).

for HIPK1 antibody (mAb)References

IF: Isono, K. et. al. (2006) Molec Cell Biol 26: 2758-2771.

for HIPK1 antibody (mAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HIPK1 antibody (mAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

HIPK1 antibody (mAb)