CHD1 antibody (pAb)

for CHD1 antibody (pAb)Background

CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

CHD1 has been shown to interact with histone H3 trimethylated at lysine 4 (H3K4Me3) and also is required for the maintenance of stem cell pluripotency.

for CHD1 antibody (pAb)Immunogen

This CHD1 antibody was raised against a recombinant protein corresponding to amino acids 251-467 of human CHD1.

for CHD1 antibody (pAb)Application Notes

This CHD1 antibody (pAb) was validated for use in Western blot and chromatin immunoprecipitaion (ChIP).
Western blot: 1:500 - 1:1,000

Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.

for CHD1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol, 0.035% sodium azide.

for CHD1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CHD1 antibody (pAb).

for CHD1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for CHD1 antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.