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SAP30 antibody (pAb)
| Catalog No: 39731 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39732 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 1:500 | — | Yes |
for SAP30 antibody (pAb) for Western blottingBackground
SAP30, also known as Sin3A-associated protein, 30kDa, is part of the SIN3 corepressor complex, that also includes SIN3, SAP18, HDAC1, HDAC2, RbAp46 and RbAp48. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.
for SAP30 antibody (pAb) for Western blottingImmunogen
This SAP30 antibody (pAb) was raised against recombinant full length human SAP30 protein.
for SAP30 antibody (pAb) for Western blottingApplication Notes
This SAP30 antibody has been validated for use in Western blotting (1:500 dilution) and immunoprecipitation. For additional information regarding this antibody, please refer to Kuzmichev et al, 2002.
for SAP30 antibody (pAb) for Western blottingBuffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for SAP30 antibody (pAb) for Western blottingPositive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CHD1 antibody (pAb).
for SAP30 antibody (pAb) for Western blottingReferences
This antibody was described in Kuzmichev, A. et al (2002) Mol Cell Biol 21: 835-848.
SAP30 antibody (pAb) tested by Western blot.
Detection of SAP30 by Western blot analysis. Lane 1: Whole cell extract of A549 cells (20 µg). Lane 2: Nuclear extract of HeLa cells (20 µg).
Both were probed with SAP30 antibody (pAb) at a 1:500 dilution.
for SAP30 antibody (pAb) for Western blottingStorage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for SAP30 antibody (pAb) for Western blottingGuarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
