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SAP30 antibody (pAb)

Aliases: Sin3A-associated protein
商品コード: 39731 Format: 100 µl ¥69,000 Buy Now
商品コード: 39732 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:30 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for SAP30 antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 µl per ChIP
ChIP-Seq & ChIP-chip: 5 µl each
WB: 1:500 dilution

for SAP30 antibody (pAb)Immunogen

This SAP30 antibody (pAb) was raised against recombinant full-length human SAP30 protein.

for SAP30 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SAP30 antibody (pAb)References

 
SAP30 antibody (pAb) tested by ChIP-Seq.

SAP30 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from the K-562 human myelogenous leukemia cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify SAP30 binding sites. The image on the left shows SAP30 binding at transcriptional start sites across a 2.4 million bp region on chromosome 1. The image on the right is zoomed in to show SAP30 binding at two other start sites.

SAP30 antibody (pAb) tested by Western blot.

SAP30 antibody (pAb) tested by Western blot.
Detection of SAP30 by Western blot analysis. Lane 1: Whole cell extract of A549 cells (20 µg). Lane 2: Nuclear extract of HeLa cells (20 µg).
Both were probed with SAP30 antibody (pAb) at a 1:500 dilution.

for SAP30 antibody (pAb)Background

SAP30, also known as Sin3A-associated protein, 30kDa, is part of the SIN3 corepressor complex, that also includes SIN3, SAP18, HDAC1, HDAC2, RbAp46 and RbAp48. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.

for SAP30 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SAP30 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SAP30 antibody (pAb).

for SAP30 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SAP30 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SAP30 antibody (pAb)
 

Data Thumbnails

SAP30 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of SAP30 pAb.
(Click image to enlarge and see details.)

SAP30 antibody (pAb) tested by Western blot.

Western blot of SAP30 pAb.
(Click image to enlarge and see details.)