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Histone H3T45ph antibody (pAb)
| RRID: AB_2793326 |
| 商品コード: 39737 | Format: 100 µl | ¥66,800 | Buy |
| 商品コード: 39738 | Format: 10 µl | ¥18,800 | Buy |
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Applications
Immunogen
This Histone H3 phospho Thr45 antibody was raised against a peptide containing phospho Thr45 of human histone H3.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
View our Guide to Histone Modifications and Biological Function.
Histone H3 phospho Thr45 antibody (pAb) tested by Western blot.
Yeast extracts from a wild type strain (Lane 1) and a strain in which threonine 45 has been mutated to an alanine (T45A, Lane 2) were blotted and probed with Histone H3 phospho Thr45 antibody (pAb) at a dilution of 1:10,000. The arrow represents the migration of histone H3.
Note that both the upper and lower bands disappear in the T45A mutant, suggesting that the upper band represents a post-translational modification of histone H3 that causes a dramatic decrease in gel mobility.
Histone H3 phospho Thr45 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr45 antibody (pAb) for Histone H3 phospho Thr45. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 phospho Thr45 antibody (pAb) at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row
Lane 1: phospho Thr45 peptide. Lane 2: unmodified Thr45 peptide. Lane 3: phospho Thr3 peptide. Lane 4: phospho Thr6 peptide. Lane 5: phospho Thr11 peptide. Lane 6: phospho Thr80 peptide. Lane 7: phospho Ser10 peptide. Lane 8: phospho Ser28 peptide.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
