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p63 antibody (pAb)

Aliases: TP63, AIS, KET, LMS, NBP, RHS, p40, p51, EEC3, OFC8, p73H, p73L, SHFM4, TP53L, TP73L, p53CP, TP53CP, B(p51A), B(p51B)
商品コード: 39739 Format: 100 µl ¥67,000 Buy Now
商品コード: 39740 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:70 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for p63 antibody (pAb)Application Notes


Validated Applications:
ChIP: 2 - 10 µl per ChIP
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP:

for p63 antibody (pAb)Immunogen

This p63 antibody was raised against a recombinant protein corresponding to full-length human p63.

for p63 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for p63 antibody (pAb)References

 
p63 antibody (pAb) tested by ChIP.

p63 antibody (pAb) tested by ChIP.
Chromatin IP was performed on chromatin from HaCaT cells. PCR primers were used to amplify a region of the FXDR gene promoter.

Lane 1: negative control IgG.
Lane 2: p63 antibody.
Lane 3: Input DNA control.

p63 antibody (pAb) tested by immunofluorescence.

p63 antibody (pAb) tested by immunofluorescence.
HaCaT cells stained with p63 antibody (pAb) (1:500 dilution).

p63 antibody (pAb) tested by Western blot.

p63 antibody (pAb) tested by Western blot.
HaCaT cell nuclear extract (20 µg per lane) was probed with p63 antibody at a dilution of 1:2,000.

for p63 antibody (pAb)Background

p63 is a sequence-specific DNA binding transcription factor that is related to the p53 protein. p63 binds to and regulates a subset of genes also regulated by p53. p63 has been postulated to have a critical role in maintaining the identity of epithelial cell layers by regulating the differentiation of epithelial stem cells. Defects in p63 are associated with a number of developmental disorders involving the limbs and palate, as well as a variety of head and neck cancers.

for p63 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for p63 antibody.

for p63 antibody (pAb)qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Hoxc10 (Cat. No. 71019) and Mouse Negative Control Primer Set 3 (Cat. No. 71013) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with p63 antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with p63 antibody (pAb).

for p63 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for p63 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

p63 antibody (pAb)
 

Data Thumbnails

p63 antibody (pAb) tested by ChIP.

ChIP of p63 pAb.
(Click image to enlarge and see details.)

p63 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of p63 pAb.
(Click image to enlarge and see details.)

p63 antibody (pAb) tested by Western blot.

Western blot of p63 pAb.
(Click image to enlarge and see details.)