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p63 antibody (pAb)
| Catalog No: 39739 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39740 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:2,000 | 2 - 10 µl per ChIP | 1:500 - 1:1,000 |
for p63 antibody (pAb)Background
p63 is a sequence-specific DNA binding transcription factor that is related to the p53 protein. p63 binds to and regulates a subset of genes also regulated by p53. p63 has been postulated to have a critical role in maintaining the identity of epithelial cell layers by regulating the differentiation of epithelial stem cells. Defects in p63 are associated with a number of developmental disorders involving the limbs and palate, as well as a variety of head and neck cancers.
for p63 antibody (pAb)Immunogen
This p63 antibody was raised against a recombinant protein corresponding to full length human p63.
for p63 antibody (pAb)Application Notes
This p63 antibody has been validated in Western blotting (1:2,000 dilution), immunofluorescence (1:500 - 1:1,000 dilution) and chromatin immunoprecipitation (ChIP) (2 - 10 µl per ChIP).
for p63 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with p63 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with p63 antibody (pAb).
p63 antibody (pAb) tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with p63 antibody at a dilution of 1:2,000.
p63 antibody (pAb) tested by immunofluorescence.
HaCaT cells stained with p63 antibody (pAb) (1:500 dilution).
p63 antibody (pAb) tested by ChIP.
Chromatin IP was performed on chromatin from HaCaT cells. PCR primers were used to amplify a region of the FXDR gene promoter.
Lane 1: negative control IgG.
Lane 2: p63 antibody.
Lane 3: Input DNA control.
for p63 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for p63 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
