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Bcl3 antibody (pAb)
| Catalog No: 39761 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39762 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for Bcl3 antibody (pAb)Background
Bcl3 (B-cell lymphoma 3 protein) functions as a transcriptional co-activator that activates through its association with NFkB homodimers. Bcl3 contains seven ankyrin repeats, which are most closely related to those found in IkB proteins. Bcl3 also functions as a form of IkB specific for the NFkB p50 subunit inhibiting its translocation to the nucleus. The expression of this gene can be induced by NFkB, which forms a part of the autoregulatory loop that controls the nuclear residence of NFkB p50. Bcl3 is overexpressed in a number of cancers, can transform fibroblasts and induce tumors in nude mice, thus implicating Bcl3 as a proto-oncogene.
for Bcl3 antibody (pAb)Immunogen
This Bcl3 antibody was raised against a peptide within the C-terminal half of human Bcl3.
for Bcl3 antibody (pAb)Application Notes
This Bcl3 antibody was validated for use in Western blotting (1:500 - 1:2,000 dilution).
for Bcl3 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Bcl3 antibody (pAb)Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Bcl3 antibody.
Bcl3 antibody tested by Western blot.
Detection of Bcl3 by Western blot analysis. Lane 1: HeLa whole cell extract (20µg). Lane 2: Nuclear extract (20µg) of Raji cells. Both probed with Bcl3 antibody at a dilution of 1:1,000.
for Bcl3 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Bcl3 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
