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Histone H3K9me3 antibody (pAb)

商品コード: 39765 Format: 100 µg ¥67,000 Buy Now
商品コード: 39766 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:17
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H3 trimethyl Lys9 antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µg per ChIP
ICC/IF: 1 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

Published Applications:
ChIP
WB
IF

See references for more information. Individual optimization may be required.

for Histone H3 trimethyl Lys9 antibody (pAb)Immunogen

This Histone H3 trimethyl Lys9 antibody was raised against a peptide including trimethyl-lysine 9 of histone H3.

for Histone H3 trimethyl Lys9 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39161) of this antibody is also available.

for Histone H3 trimethyl Lys9 antibody (pAb)References

 
Histone H3K9me3 antibody (pAb) tested by ChIP.

Histone H3 trimethyl Lys9 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µg of Histone H3 trimethyl Lys9 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H3K9me3 antibody (pAb) tested by immunofluorescence.

Histone H3 trimethyl Lys9 antibody tested by immunofluorescence.
Detection of Histone H3 trimethyl Lys9 by immunofluorescence. HeLa cells were stained with Histone H3 trimethyl Lys9 antibody at a dilution of 1 µg/ml. Top panel: Histone H3 trimethyl Lys9 antibody staining. Middle panel: DAPI. Bottom panel: merge.

Histone H3K9me3 antibody (pAb) tested by Western blot.

Histone H3 trimethyl Lys9 antibody tested by Western blot.
HeLa nuclear extract (20 µg) was probed with Histone H3 trimethyl Lys9 antibody (1 µg/ml dilution).

Histone H3K9me3 antibody (pAb) tested by dot blot analysis.

Histone H3 trimethyl Lys9 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of 39765 for trimethyl-lysine 9 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with 39765 at a dilution of 1 µg/ml. The amount of peptide (in picomoles) spotted is indicated next to each row.
Top row Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: dimethyl Lys14. Lane 10: monomethyl Lys18. Lane 11: dimethyl Lys18. Lane 12: trimethyl Lys18. Bottom row, Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36.

for Histone H3 trimethyl Lys9 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

for Histone H3 trimethyl Lys9 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 trimethyl Lys9 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K9me3 antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K9me3 antibody (pAb).

for Histone H3 trimethyl Lys9 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 trimethyl Lys9 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K9me3 antibody (pAb)
 

Data Thumbnails

Histone H3K9me3 antibody (pAb) tested by ChIP.

ChIP of Histone H3K9me3 pAb.
(Click image to enlarge and see details.)

Histone H3K9me3 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H3K9me3 pAb.
(Click image to enlarge and see details.)

Histone H3K9me3 antibody (pAb) tested by Western blot.

Western blot of Histone H3K9me3 pAb.
(Click image to enlarge and see details.)

Histone H3K9me3 antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K9me3 pAb.
(Click image to enlarge and see details.)