Estrogen Receptor β antibody (mAb)

RRID: AB_2793335
Clone: CO1531
Aliases: ESR2, Erb, ESRB, ESTRB, NR3A2, ER-BETA, ESR-BETA
Catalog No: 39767 Format: 100 µg ¥86,000 Buy
Catalog No: 39768 Format: 10 µg ¥24,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
59 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunohistochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP

See the original reference for clone CO1531, which has been published for IP, WB, and IHC.

Immunogen

This Estrogen Receptor beta antibody was raised against a recombinant protein corresponding to amino acids 212-477 of human Estrogen Receptor beta.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

ERβ (estrogen receptor β) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. ERβ localizes to the nucleus where it may form a homodimer or a heterodimer with ER alpha. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer and osteoporosis.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting