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Estrogen Receptor β antibody (mAb)

Clone: CO1531
Aliases: ESR2, Erb, ESRB, ESTRB, NR3A2, ER-BETA, ESR-BETA
商品コード: 39767 Format: 100 µg ¥67,000 Buy Now
商品コード: 39768 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:59 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunohistochemistry Validated

for Estrogen Receptor b antibody (mAb) (Clone CO1531)Application Notes


Published Applications:
IP
WB
IHC
See references for more information. Individual optimization may be required.

for Estrogen Receptor b antibody (mAb) (Clone CO1531)Immunogen

This Estrogen Receptor beta antibody was raised against a recombinant protein corresponding to amino acids 212-477 of human Estrogen Receptor beta.

for Estrogen Receptor b antibody (mAb) (Clone CO1531)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Estrogen Receptor b antibody (mAb) (Clone CO1531)References

for Estrogen Receptor b antibody (mAb) (Clone CO1531)Background

ERβ (estrogen receptor β) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. ERβ localizes to the nucleus where it may form a homodimer or a heterodimer with ER alpha. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer and osteoporosis.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Estrogen Receptor β antibody (mAb).

for Estrogen Receptor b antibody (mAb) (Clone CO1531)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Estrogen Receptor b antibody (mAb) (Clone CO1531)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Estrogen Receptor β antibody (mAb)