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E1A antibody (mAb)
| Clone: M73 |
| Catalog No: 39771 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39772 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 0.1 - 1 µg/ml | — | 1 - 5 µg/ml | 1 - 5 µg per IP |
for E1A antibody (mAb) (Clone M73)Background
Adenovirus E1A protein is a transcriptional activator of adenovirus early promoters. This E1A antibody recognizes E1A from cells infected with Adenovirus 2 or Adenovirus 5, and recognizes three major bands from 30 - 50 kDa in molecular weight.
for E1A antibody (mAb) (Clone M73)Immunogen
This E1A antibody was raised against a recombinant protein corresponding to full length E1A from Adenovirus 2.
for E1A antibody (mAb) (Clone M73)Application Notes
This E1A antibody has been validated for use in Western blot (0.1 - 1 µg/ml), immunofluorescence (1 - 5 µg/ml) and immunoprecipitation (1 - 5 µg/ml).
for E1A antibody (mAb) (Clone M73)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use E1A antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with E1A antibody (mAb).
for E1A antibody (mAb) (Clone M73)References
This antibody is described in Harlow, E. et al (1985) J Virol 55: 533-546.
This antibody was used in the various applications as described:
Immunoprecipitation: Cook, J. et al (1996) Proc Natl Acad Sci USA 93: 13985 - 13990.
Immunofluorescence / Immunocytochemistry: Madison, D. et al (2002) J Biol Chem 277: 38755 - 38763.
Western blotting: Cook, J. et al (1996) Proc Natl Acad Sci USA 93: 13985 - 13990.
E1A antibody tested by Western blot.
HEK293 whole cell extract probed with E1A antibody (Clone M73) (0.1 μg/ml dilution). Arrows indicate the Ad2 and Ad5 E1A gene products.
for E1A antibody (mAb) (Clone M73)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for E1A antibody (mAb) (Clone M73)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
