Drosha antibody (pAb)

for Drosha antibody (pAb)Background

Drosha is an RNAse III enzyme that is involved in the process of generating microRNA (miRNA). miRNA interacts with the RNA-induced silencing complex (RISC) to cleave mRNA and thus downregulate gene expression. Drosha is a double stranded RNAse and part of the Microprocessor complex. Other RNAse III enzymes include Dicer and Argonaute. Drosha initiates processing of the miRNA precursor (pri-miRNA) in the nucleus, which is then exported to the cytoplasm and further cleaved by Dicer into a mature miRNA.

for Drosha antibody (pAb)Immunogen

This Drosha antibody was raised against a peptide within the C-terminal region of human Drosha.

for Drosha antibody (pAb)Application Notes

This Drosha antibody has been validated for use in Western blotting (1:500 - 1:2,000).

Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.

for Drosha antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Drosha antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Drosha antibody (pAb).

for Drosha antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Drosha antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.