Drosha antibody (pAb)

RRID: AB_2793342
Aliases: RN3, ETOHI2, RNASEN, RANSE3L, RNASE3L, HSA242976
Catalog No: 39783 Format: 100 µl ¥86,000 Buy
Catalog No: 39784 Format: 10 µl ¥24,000 Buy

Request a quote for a bulk order Request Quote


Product Review Submit a Review


Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
160 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes

Applications Validated by Active Motif:
IP: 10 µl per IP
WB*: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Immunogen

This Drosha antibody was raised against a peptide within the C-terminal region of human Drosha.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Drosha antibody (pAb) tested by Immunoprecipitation.
10 µl of Drosha antibody was used to immunoprecipitate Drosha from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Drosha antibody at a dilution of 1:500.

Drosha antibody (pAb) tested by Western blot.
HeLa cell nuclear extract (40 µg) probed with Drosha antibody at a dilution of 1:500.

Background

Drosha is an RNAse III enzyme that is involved in the process of generating microRNA (miRNA). miRNA interacts with the RNA-induced silencing complex (RISC) to cleave mRNA and thus downregulate gene expression. Drosha is a double stranded RNAse and part of the Microprocessor complex. Other RNAse III enzymes include Dicer and Argonaute. Drosha initiates processing of the miRNA precursor (pri-miRNA) in the nucleus, which is then exported to the cytoplasm and further cleaved by Dicer into a mature miRNA.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Drosha antibody.

MicroRNA-3´UTR Interaction Products

If you are interested in this antibody, you may also have an interest in products for studying the functional impact of miRNA-3´UTR interactions. The LightSwitch™ 3´UTR Reporter GoClone™ Collection includes over 12,000 human 3´UTRs that have been cloned into the pLightSwitch_3UTR vector for use in luciferase reporter assays. These constructs can be co-transfected with our collections of LightSwitch miRNA Mimics and Inhibitors to study the effects of over-expression of a miRNA of interest, or to knock down the expression of endogenous human miRNAs in living cells.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting