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MITF antibody (mAb)
| Catalog No: 39789 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39790 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | ChIP-Seq / ChIP-chip |
IF | IP |
|---|---|---|---|---|
| 0.5 - 2 μg/ml | 2.5 - 10 µg per ChIP | 4 µg per ChIP-Seq 4 µg per ChIP-chip |
Published. See Application Notes below. |
10 µg per IP |
for MITF antibody (mAb)Background
MITF protein (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix type transcription factor involved in the differentiation and development of retinal pigment epithelium and melanocytes. It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. For activity MITF needs to heterodimerize with other bHLH proteins such as TFE3, TFEB or TFE. Defects in MITF are the cause of Waardenburg syndrome type 2A and Tietz syndrome.
for MITF antibody (mAb)Immunogen
This MITF antibody was raised against an N-terminal fragment of human MITF.
for MITF antibody (mAb)Application Notes
This MITF antibody has been validated for use in chromatin IP (ChIP, 2.5 - 10 µg per ChIP), ChIP-Seq & ChIP-chip (4 µg each), EMSA (0.5 - 2 µg per ml), immunofluorescence (IF, see References for details), immunohistochemistry and immunoprecipitation (10 µg per IP).
for MITF antibody (mAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with MITF antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use MITF antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with MITF antibody (mAb).
for MITF antibody (mAb)References
This antibody has been used as described below:
EMSA: Du, J. et al (2003) Am J Path 163: 333-343.
IF: Wellbrock, C. and Marais, R. (2005) J Cell Biol 170: 703-708.
IP: Hemesath, P. et al (1998) Nature 391: 298-301.
WB: Wellbrock, C. and Marais, R. (2005) J Cell Biol 170: 703-708; Hemesath, P. et al (1998) Nature 391: 298-301.
MITF antibody (mAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using Active Motif's Epigenetic Services protocol with 30 µg of chromatin from human lung melanoma cell line MALME-3M and 4 µg of MITF antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
MITF antibody (mAb) tested by ChIP-Seq.
ChIP was performed using chromatin from a human melanoma cell line (2.5 million cells). ChIP DNA was sequenced on the Illumina GA II and 19 million sequence tags were mapped to identify MITF binding sites across the genome. The image shows hundreds of MITF binding sites across a 60 million bp region on chromosome 9.
for MITF antibody (mAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for MITF antibody (mAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
