HDAC9 antibody (pAb)

for HDAC9 antibody (pAb)Background

HDAC9 (Histone Deacetylase 9) is a class II mammalian histone deacetylase (HDAC) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). In humans, expression of HDAC9 is expresses in a wide variety of tissues. It interacts with HDAC1, HDAC3, and probably with HDAC4 and HDAC5. It also interacts with CTBP1, MEF2, MAPK10, ETV6, NCOR1 and BCL6.

for HDAC9 antibody (pAb)Immunogen

This HDAC9 antibody was raised against a peptide derived from the C-terminus of human HDAC9.

for HDAC9 antibody (pAb)Application Notes

This HDAC9 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution). This antibody recognizes isoform 4 & 5 of HDAC9 and not isoform 1 or 3

for HDAC9 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for HDAC9 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36053) can be used as a positive control for HDAC9 antibody.

for HDAC9 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HDAC9 antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.