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MEIS 1/2/3 antibody (mAb)

Clone: 9.2.7
商品コード: 39795 Format: 100 µg ¥67,000 Buy Now
商品コード: 39796 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:54-66 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Electrophoretic Mobility Shift Assay Validated Immunocytochemistry Validated

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Application Notes


Validated Applications:
ICC/IF: 0.5 µg/ml dilution

Published Applications:
ChIP
WB
IHC
EMSA
See references for more information. Individual optimization may be required.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Immunogen

This MEIS antibody was raised against a recombinant protein corresponding to amino acids amino acids 50-390 mouse MEIS1.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)References

 
MEIS 1/2/3 antibody (mAb) tested by immunofluorescence.

MEIS 1/2/3 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with MEIS 1/2/3 antibody at a 0.5 µg/ml dilution.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Background

The MEIS family or proteins (Myeloid Ecotropic viral Integration Site homologues) encode proteins that function as cofactors for the Homeobox (HOX) proteins. Homeobox genes play a crucial role in normal development. In addition, several homeoproteins are involved in neoplasia. MEIS and a functionally related protein Pbx belong to the TALE (Three Amino acid Loop Extension) homeodomain superfamily of proteins. The role of MEIS proteins in the regulation of transcription is as yet unclear.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with MEIS 1/2/3 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use MEIS 1/2/3 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with MEIS 1/2/3 antibody (mAb).

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MEIS 1/2/3 antibody (mAb)
 

Data Thumbnails

MEIS 1/2/3 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of MEIS 1/2/3 mAb.
(Click image to enlarge and see details.)