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Antibodies

MEIS 1/2/3 antibody (mAb)

Clone: 9.2.7
Catalog No: 39795 Format: 100 µg $340 Buy Now
Catalog No: 39796 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Protein G Chromatography
Applications:ChIP, EMSA, IHC, IP, WB
Reactivity:Human, Mouse
Molecular Weight:54-66 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
0.5 - 2 µg/ml 2 - 10 µg per ChIP Published.
See Application Notes below.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Background

The MEIS family or proteins (Myeloid Ecotropic viral Integration Site homologues) encode proteins that function as cofactors for the Homeobox (HOX) proteins. Homeobox genes play a crucial role in normal development. In addition, several homeoproteins are involved in neoplasia. MEIS and a functionally related protein Pbx belong to the TALE (Three Amino acid Loop Extension) homeodomain superfamily of proteins. The role of MEIS proteins in the regulation of transcription is as yet unclear.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Immunogen

This MEIS antibody was raised against a recombinant protein corresponding to amino acids amino acids 50-390 mouse MEIS1.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Application Notes

This MEIS 1/2/3 antibody recognizes MEIS1, MEIS2 and MEIS3 proteins.

This MEIS antibody has been validated for use in chromatin immunoprecipitation (ChIP, 2 - 10 µg per ChIP), EMSA (2 µg per assay), immunohistochemistry (IHC, 2 - 10 µg/ml), immunoprecipitation, and Western blot (0.5 - 2 µg/ml). See references for more details on specific applications.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with MEIS 1/2/3 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use MEIS 1/2/3 antibody (mAb).

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)References

This antibody was used in applications as described below
ChIP: Deramaudt, T.B. et al (2006) J Biol Chem 281: 38385-38395; Shim, S. et al (2007) Mol Cell Biol 27: 1614-1630.
EMSA: Shim, S. et al (2007) Mol Cell Biol 27: 1614-1630; Rave-Harel, N. et al (2004) J Biol Chem 279: 30287-30297.
IHC: Shim, S. et al (2007) Mol Cell Biol 27: 1614-1630.
WB: Fiskus, W. (2006) Mol Cancer Ther 5: 3096-3104; Rave-Harel, N. et al (2004) J Biol Chem 279: 30287-30297; Shim, S. et al (2007) Mol Cell Biol 27: 1614-1630

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

MEIS 1/2/3 antibody (mAb)