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AIB1 / SRC-3 antibody (pAb)
| Catalog No: 39797 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39798 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | 10 µl per ChIP |
for AIB1 / SRC-3 antibody (pAb)Background
AIB1 (Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3) is a chromatin-associated protein that interacts with nuclear hormone receptors to modify their activity as transcriptional activators. AIB1 has histone acetyltransferase (HAT) activity and recruits p300/CBP-associated factor (PCAF) and CREB binding protein (CBP) as part of a multisubunit co-activator complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. It is Involved in the coactivation of different nuclear receptors, such as GR, ER, RARs, RXRs, TRs, VDR and PPARs. It is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 protein.
for AIB1 / SRC-3 antibody (pAb)Immunogen
This AIB1 antibody was raised against a peptide derived from human AIB1/SRC-3.
for AIB1 / SRC-3 antibody (pAb)Application Notes
This AIB1 / SRC-3 antibody has been validated for use in Western blotting (1:500 - 1:2,000) and chromatin IP (10 µl per ChIP).
for AIB1 / SRC-3 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for AIB1 / SRC-3 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36053) can be used as a positive control for HDAC9 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with AIB1 / SRC-3 antibody (pAb).
AIB1 antibody (pAb) tested by Western blot.
Detection of AIB1 by Western blot. Lane 1: HeLa whole cell extract (30 µg). Lane 2: Whole cell extract (30 µg) of MCF7 cells. Both probed with AIB1 antibody at a 1:500 dilution.
AIB1 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and MCF7 chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of AIB1 / SRC-3 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the pS2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for AIB1 / SRC-3 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for AIB1 / SRC-3 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
