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Myf-5 antibody (mAb)
| Clone: 1E2G4 |
| Catalog No: 39801 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39802 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 0.5 - 2 μg/ml | — | 1 µg/ml |
for Myf-5 antibody (mAb) (Clone 1E2G4)Background
Myf-5 (Myogenic Factor 5, MFY5) is a basic helix-loop-helix transcription factor involved in muscle differentiation. Myf-5 induces differentiation of fibroblasts into myoblasts. Efficient DNA binding requires dimerization with another bHLH protein.
for Myf-5 antibody (mAb) (Clone 1E2G4)Immunogen
This Myf-5 antibody was raised against a peptide derived from mouse Myf-5.
for Myf-5 antibody (mAb) (Clone 1E2G4)Application Notes
This Myf-5 antibody has been validated for use in immunofluorescence (IF, 1 µg per ml dilution) and Western blot (0.5 - 2 µg per ml dilution).
for Myf-5 antibody (mAb) (Clone 1E2G4)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Myf-5 antibody (mAb).
for Myf-5 antibody (mAb) (Clone 1E2G4)References
This antibody was used in immunofluorescence and Western blot as described below:
Harada, A. et al (2009) Hybridoma 28: 451-453.
for Myf-5 antibody (mAb) (Clone 1E2G4)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Myf-5 antibody (mAb) (Clone 1E2G4)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
